Confocal imaging capacity on a widefield microscope using a spatial light modulator
Fig 5
SLM improves sectioning capability of localized fluorescent source.
(A) Average transverse intensity profile of 6-μm fluorescent beads. Dotted line at 10% maximum intensity. Insets show maximum projection of average bead images with profile line. (B) Average axial intensity profile of 6-μm fluorescent beads. Insets show maximum projection of average bead images with profile line. (C) Transverse full width at 10% maximum intensity in (A). (D) Axial full width at 10% maximum intensity in (B). Note improved confinement of fluorescence to true bead extent in SLM imaging compared to widefield, particularly in axial direction. n = 85 (widefield), 90 (SLM-pick), and 63 (confocal) beads.