Confocal imaging capacity on a widefield microscope using a spatial light modulator
Fig 2
Illumination setup and resolution.
(A) SLM on 3D-printed mount on 6-axis fine-positioning stage. SLM inserted into field stop of microscope. (B) Transverse illumination resolution determined using fluorescent dye thin film. (C) Approximate widefield axial illumination beam and intensity profiles. Illumination spans FOV (red). (D) Approximate scanned axial illumination beam and intensity profiles. Beam is scanned across FOV (red line). (E) Measured intensity along optical axis for widefield and SLM-scanned illumination beams. SLM-scanned illumination (black) has significantly better resolution than widefield illumination (gray).