Characterization of ecotin homologs from Campylobacter rectus and Campylobacter showae
Fig 5
E. coli and Campylobacter ecotins possess similar IC50 values for neutrophil elastase.
Experimentally determined IC50 values (nM) for E. coli (circles, 4.64 ± 0.23), C. rectus (squares, 4.49 ± 0.25) and C. showae (triangles, 4.78 ± 0.31) ecotins used at increasing concentrations (nM) to inhibit NE. Each data point represents the mean from three independent measurements. Relative florescence (in %, where 100% indicates fully digested FRET-peptide and 0% indicates fully inhibited protease) was determined after measuring the samples in a microplate reader with a filter set of Ex/Em = 355/530 nm. Separate graphs for each IC50 determination were included in the supplementary information (S5 Fig).