Development and validation of a NanoString BASE47 bladder cancer gene classifier
Fig 2
Development of a NanoString BASE47 classifier that performs on NanoString derived RNA expression values.
RNA from UNC-Training set samples (n = 52) were profiled by both RNAseq (RNA Access) and NanoString nCounter assay for the BASE47 probeset to generate RNAseq and NanoString expression matrices respectively. “True” biologic calls were assigned by applying the previously published Transcriptome BASE47 classifier to the RNAseq data. (A) Application of the Transcriptome BASE47 classifier to the NanoString expression data resulted in a 46% misclassification error. (B) Application of the newly derived NanoString BASE47 classifier to NanoString expression data resulted in only 13% misclassification error (relative to the “True” biologic subtype designations).