Optimised generation of iPSC-derived macrophages and dendritic cells that are functionally and transcriptionally similar to their primary counterparts
Fig 5
iPSC-derived macrophages and MDM show similar transcriptional and activation profiles.
(A) Correlation scatter plot of the average rlog expression values for MDM versus iPSdM. Only genes with TPM>2 in either group are shown. Genes with fold change >4 in green and genes with fold change < -4 in blue. (B) Summary of functional groups enriched for the 1964 DE genes. Bar length is the -log2 of the enrichment p-value and the colour represents the z-score with green indicating functions activated in iPSdM relative to MDM and blue bars those activated in MDM relative to iPSdM. (C) Heatmap illustrating changes in expression of 10 LPS inducible genes (left) for both MDM and iPSdM following LPS stimulation. (D) PCA plot iPSdM and MDM samples with and without LPS stimulation. Grey arrows show the progression from no LPS to 1h LPS to 6h LPS.