Toward a better definition of hematopoietic progenitors suitable for B cell differentiation
Fig 5
Gene expression at different time point of B cell differentiation.
Gene expression analysis by Taqman expression assay of PAX5, EBF1, VPREB1, IL-7RA, IGLL1, RAG1 and OCT4 were performed on total cells at different time point during B cell differentiation. Histograms of 2-DDCT value display gene expression analysis of cells (A) at Day 21 of B cell differentiation from UCB CD34+ cells (n = 2 to 3 independent experiments) and (B) at Day 0 (D0), 7, 14 and 21 of B cell differentiation from hESC-derived CD34+ with undifferentiated hESC and tonsil B cells (LB) as controls (n = 1 to 2 independent experiments).