Structure of the pecten neuropil pathway and its innervation by bimodal peg afferents in two scorpion species
Fig 3
Experimental set-up for anterograde tracing of peg afferents.
(A) Photo of a cold-anaesthetised Heterometrus petersii fixed to a cork board, ventral side up. Pectines were cleaned, stabilised with plasticine, fixed into position with dental glue and supported with a plastic film. (B) Sketch of the anterograde tracing procedure using Neurobiotin. Pectines are highlighted in beige, examples of incisions at individual pegs in different positions marked in red. Glass capillaries filled with Neurobiotin were put over the incision. Abbreviations: a: anterior; p: posterior.