Improved cryopreservation of in vitro produced bovine embryos using FGF2, LIF, and IGF1
Fig 5
FLI supplementation advances transzonal projection dissociation.
(A) The effect of the addition of FLI to oocyte maturation medium on the number of TZP at 6, 12, 18, and 24hr. (n = 198; 100 control and 98 +FLI). (B) The images are representative of TZP dissociation at 24hr of maturation. Arrows indicate intact TZP. Asterisk (*) indicates statistical differences (P < 0.05).