Regulation of sperm motility in Eastern oyster (Crassostrea virginica) spawning naturally in seawater with low salinity
Fig 5
Effect of potassium (K+) ions on sperm motility (%, A-C) and velocity (μm/s, D-F) in Eastern oyster, Crassostrea virginica. Sperm was activated in K+-free artificial seawater (ASW) and ASW containing a voltage-gated (4-aminopyridine, 4-AP) or an ATP-sensitive (glybenclamide, G) K+ channel blocker. Average motility in each treatment (B) and time post-activation (C) is displayed. Data were analyzed using a repeated measures ANOVA and shown as mean ± SE (n = 5). Treatments with different superscripts significantly differ (p < 0.05).