Assessment of microbiological correlates and immunostimulatory potential of electron beam inactivated metabolically active yet non culturable (MAyNC) Salmonella Typhimurium
Fig 5
EBST remains metabolically active and retains the activity for extended time periods of storage at 4°C.
Metabolic activity of S. Typhimurium post eBeam irradiation was measured using Alamar blue assay. The reduced environment present in metabolically active cells are detected by redox indicator which fluoresces. The fluorescence readings obtained for EBST, live ST and heat killed ST (HKST) was measured on a daily basis up to 9 days and are reported as line graph. Metabolic activity of EBST cells followed by heat inactivation (EBST+HK) and HKST followed by eBeam irradiation (HKST+EB) were measured as process controls.