Interaction of amphiphilic lipoarabinomannan with host carrier lipoproteins in tuberculosis patients: Implications for blood-based diagnostics
Fig 5
Comparison of lipoprotein capture and membrane insertion.
(a) Representative measurement of LAM (0.5 μM), by lipoprotein capture (black bars) and membrane insertion assay (grey bars), incubated overnight at 4 °C in control human serum, with the specific signal (RFU) from the detection of α-LAM antibody (15 nM). Values are the mean ± standard deviation derived from at least two independent determinations (n = 2). Statistical significance was determined by Welch’s t-test (*P < 0.05). (b) Comparison of LAM detection signal by lipoprotein capture (black bars) and membrane insertion assay (grey bars) in patient serum samples. Data are presented as the S/N ratio with a value over 1 indicating a positive result. Samples 14, 3, 4, 27, 22 and 24 are positive for LAM by either blood or sputum culture methods, whereas samples 52, 53 are healthy controls.