Identification of a new myotropic decapeptide from the skin secretion of the red-eyed leaf frog, Agalychnis callidryas
Fig 2
Sepration and identification of callitide mature peptide.
(A) Region of reverse phase HPLC chromatogram of Agalychnis callidryas skin secretion. The arrow indicates the elution position of peak (#35) containing the myotropic decapeptide callitide; (B) LCQ mass spectrum of callitide from the skin secretion of Agalychnis callidryas. Ions at m/z 598.94 and 399.81 represent doubly charged (M+2H)2+ and triply charged (M+3H)3+ ions, respectively; (C) Electrospray ion-trap MS/MS fragmentation datasets derived from ions corresponding in molecular mass to callitide. Expected singly-, doubly- and triply- charged b-ion and y-ion fragment m/z ratios were predicted and observed fragment ions are indicated in bold type-face and are underlined; (D) MALDI-TOF mass spectrum of synthetic copy of callitide.