Dissecting seed pigmentation-associated genomic loci and genes by employing dual approaches of reference-based and k-mer-based GWAS with 438 Glycine accessions
Fig 3
Genomic structures and gene expression profiles around the k-meri allele.
(A) Comparative genomic structures between G. soja (PI483463) and G. max (Williams 82 BAC77G7-a) at the I-cluster B. (B) Gene expression profile and Sashimi plot of chimeric subtilisin-CHS1/CHS3 gene. X-axis indicates relative genomic locations within the BAC77G7-a BAC clone, while Y-axis the read depth. Solid lines are used to depict exon/intron junction-to-junction, and corresponding number of reads that are splitted due to the junctions are denoted on each line. The upper part of Sashimi plot demonstrates mapping result of mRNA reads with an emphasis on distribution of junction-spanning reads along on the chimeric subtilisin-CHS1/CHS3. The dark and light colors in arrow-shaped boxes represent the exons and introns of the gene, respectively.