Heterogeneous expression of CFTR in insulin-secreting β-cells of the normal human islet
Fig 5
Cftr contributes to the insulin secretory response in mouse, rat and human islets and the MIN6 β-cell line.
A. Proof of specificity for CFTRinh-172 (0–10μM) on the secretory response using islets obtained from transgenic mice lacking Cftr (CftrKO) and CftrWT mice, in response to 5.5mM and 12.5mM glucose (n = 8, *p<0.05). B, C. Effect of 5μM CFTRinh-172 on insulin secretion of mouse CftrKO and CftrWT islets (B, n = 5, *p<0.05) and rat islets (C, n = 3, *p<0.05) in response to 5.5mM and 12.5mM glucose. D. Basal (5.5mM glucose) and stimulated (12.5mM glucose) insulin secretory response of freshly isolated primary human islets in the presence of vehicle (DMSO) or 5μM CFTRinh-172 (n = 5 donors, *p<0.05). E. Dose-response curve of basal (5.5mM glucose) and stimulated (12.5mM glucose) insulin secretion from human islets (n = 4 donors, *p<0.05) treated with the indicated concentrations of CFTRinh-172. F. Basal (5.5mM glucose) and stimulated (12.5mM glucose) insulin secretory response of MIN6 β-cells incubated with vehicle (DMSO) or 5μM Inh172 (n = 3, *p<0.05).