Identification of the enzymes responsible for m2,2G and acp3U formation on cytosolic tRNA from insects and plants
Fig 5
CG10050 encodes the DTWD2A enzyme in D. melanogaster.
(A) Sequence alignment of DTWD2 proteins. Green box represents the DTW domain, and arrows denote predicted active site residues. (B) Schematic of D. melanogaster tRNAVal(CAC). Location of primer binding is shown in blue. Selected nucleotides are numbered in red. (C) Knockdown of CG10050 by RNAi results in loss of a primer extension block consistent with acp3U20b on tRNA. S2R+ cells were treated three times over 8 days with dsRNA to indicated genes. After harvest of cells, RNA was extracted and primer extension to tRNAVal(CAC) was performed.