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Identification of the enzymes responsible for m^2,2G and acp³U formation on cytosolic tRNA from insects and plants

Fig 2

Detection of m^2,2G₂₆ by fluorescent primer extension in yeast cells.

(A) Schematic of S. cerevisiae tRNA^Tyr. Location of primer binding is shown in blue. Selected nucleotides are numbered in red. (B) Detection of m^2,2G₂₆ by fluorescent primer extension in yeast cells. Left, bulk RNA was extracted from indicated strains and analyzed by primer extension to yeast tRNA^Tyr. Selected nucleotides from the sequencing reactions are provided on the left of the gel. Right, bulk RNA was extracted from culture of indicated individual strains or culture from mixtures of strains in indicated ratios, and then analyzed by primer extension to yeast tRNA^Tyr.

doi: https://doi.org/10.1371/journal.pone.0242737.g002