Identification of the Clostridial cellulose synthase and characterization of the cognate glycosyl hydrolase, CcsZ
Fig 5
The product-bound structure of CcsZ.
(A) A glucose molecule is bound at the apparent -2 subsite adjacent the catalytic architecture and confined by the orientation of Y237. (B) The bound glucose molecule makes polar contacts with W314 via the C1 hydroxyl and N48 via the C2 hydroxyl and H123 via the C6 hydroxyl. (C) The polder omit map electron density (blue mesh) for the glucose ligand contoured at 3 σ.