Characterization of binding between model protein GA-Z and human serum albumin using asymmetrical flow field-flow fractionation and small angle X-ray scattering
Fig 5
SEC-SAXS data and modeling of the GA-Z dimer.
(a) Experimental data is shown as white circles with the fits using Coral (red line) and EOM (dashed blue line) modeling. (b) Coral rigid body model results corresponding to the fit in (a), superimposed on the ab initio model using DAMMIF on the same experimental data. The model is shown also after a 90 ° rotation. (c) Results from the EOM modeling, shown in grey filling is the random pool from which models could be chosen to fit the data in (a). The black line corresponds to the frequency of models with a certain radius of gyration that was used in the final fit in (a) to the experimental data. (d) Four representative models from the EOM modeling procedure, shown in ribbon representation.