The DARC-null trait is associated with moderate modulation of NK cell profiles and unaltered cytolytic T cell profiles in black South Africans
Fig 2
NK cell subset frequencies and markers of differentiation.
(A) Total NK cells grouped by relative CD56 and CD16 expression into CD56 bright, CD56 dim and CD56 negative subsets. (B) NK cell subset frequencies in all participants. NK cell subset frequencies stratified by DARC genotype in HIV negative (C) and HIV positive (D) individuals. (E) Gating strategy for CD57 expressing NK cells as a percentage of total NK cells. (F) CD57 expressing NK cells as a percentage of total NK cells in all participants. CD57 expressing NK cells as a percentage of total NK cells stratified by DARC genotype in HIV negative (G) and HIV positive (H) individuals. Correlations between CD57 expressing NK cells and ANCs are shown in HIV negative (I) and HIV positive (J) individuals. (K) Representative strategy employed for the identification of KIR and/or NKG2A expressing NK cells. (L) KIR/NKG2A subsets as a percentage of total NK cells in all participants. KIR/NKG2A subsets further stratified by DARC genotype in HIV negative (M) and HIV infected (N) individuals. Dots indicate individual data points. Medians are indicated and extended to interquartile range with whiskers. Abbreviations: DARC, Duffy antigen receptor for chemokines; K+N-, KIR+NKG2A- NK cell subset; K+N+, KIR+NKG2A+ NK cell subset; K-N+, KIR-NKG2A+ NK cell subset; K-N-, KIR-NKG2A- NK cell subset.