Demonstration of a fast and easy sample-to-answer protocol for tuberculosis screening in point-of-care settings: A proof of concept study
Fig 2
Representative traces of the qPCR amplification of M. tuberculosis IS6110 target.
Porcine mucin samples spiked with M. tuberculosis H37Rv DNA (25 ng/μL) were processed according to protocols #3 or #4. Traces “a” and “b” were obtained after 1:10 and 1:100 dilution of the eluted DNA obtained with protocol #3. Traces “c” and “d” were obtained after 1:10 and 1:100 dilution of the eluted DNA obtained with protocol #4. “PC” and “NC” represent the positive control (25 ng/μL of DNA extracted from H37Rv MTB cells) and the negative control (TE pH 8.0), respectively. Traces “e”, “f”, “g”, and “h”, (“e–h”) represent 1:100 dilutions of protocols #1, #2, #5, and #6. Traces are representative of at least three independent extraction procedures.