Context-dependent monoclonal antibodies against protein carbamidomethyl-cysteine
Fig 5
Detection of protein CAM-cys after pulse chase labeling of living cells with IAM.
IAM (1mM) was added to growth media of 293 cells for 2 minutes followed by media without IAM for defined periods of time. Chase time intervals (lanes marked by triangle t) were 0, 8, 24, and 32 hours. Cells were collected for analysis of labeled protein content by Western blotting with 4E7 (A) and 52H11 (B). Western blotting for tubulin (TUB) served as a control for total protein loading.