Evaluation of metronomic chemotherapy response using diffusion and dynamic contrast-enhanced MRI
Fig 2
Quantification of immature vessels that lack COLIV staining for basement membrane.
(A) A representative patch (500 x 500 pixels; 0.2 x 0.2 mm2) with vessels stained positive mostly for both CD31 and COLIV, which has a high covariance between the two staining density maps. (B) A representative patch with vessels that has strong staining for CD31, but weak staining for COLIV. This case has a lower covariance between the two staining density maps. (C) The whole slice CD31 and COLIV stained images for a case in the control group. (D) The CD31 and COLIV density maps estimated from the corresponding IHC images shown in (C). The immature vessel density map shown in the last column was generated using the stain covariance map between CD31 and COLIV as a mask to show CD31 densities only for the patches with low covariances.