Bacterial genome editing by coupling Cre-lox and CRISPR-Cas9 systems
Fig 2
Construction of Cas9-mediated S. oneidensis flaG gene deletion mutants.
(A): The editing schematic diagram and screening primers are shown for deletion of the flaG gene. (B): The phenotype of different S. oneidensis strains. WT, wild type. Cas9, the wild type strain harboring Cre, Cas9, RecET and AprR in the chromosome. Cas9+sgRNA, the wild type strain harboring donor template, sgRNA, KanR, Cas9, RecET and AprR in the chromosome. (C): PCR screening of the initial transconjugant colonies. L: DNA ladder. WT: wild type as positive control. 1–16: 16 randomly picked colonies.