Blood-brain barrier permeability analysis of plant ceramides
Fig 3
In vitro Blood-Brain Barrier (BBB) permeability.
(A) Schematic representation of an in vitro BBB model constructed from primary cultures of rat brain capillary endothelial cells, brain pericytes, and astrocytes. Synthetic plant-type ceramides (5 μM) were added to the medium in the inserts. After 30 min, conditioned medium in the bottom wells was collected and analyzed by LC-MS/MS. (B) Chemical structure of d18:2/C5-ceramide. (B) Chemical structures of d18:2/C6- and d18:2/C15-ceramides. (C) Levels of d18:2/C6-ceramide and d18:2/C15-ceramide. Data are presented as means ± SD (n = 3; *p <0.05 by t-test. (D) Levels of d18:2/C6-SM, d18:2/C15-SM, and GlcCer. Data are represented as means ± SD (n = 3; ns, not significant; *p <0.05 by t-test).