Bergenin protects pancreatic beta cells against cytokine-induced apoptosis in INS-1E cells
Fig 4
Bergenin suppresses cytokine-induced apoptosis in beta cells.
INS-1E cells were treated with cytokines (IL-1β, INF-γ, and TNF-α) in the presence or absence bergenin (2–10 μM) for 48 hr, and were assessed for apoptosis inhibition. (A) Represents the flow cytometric analysis of INS-1E cells treated with bergenin in the presence of cytokines cocktail. The viable cell populations are in the lower left quadrant (Annexin V-/PI-); the cells at the early apoptosis are in the lower right quadrant (Annexin V+/PI-); and the ones at the late apoptosis are in the upper right quadrant (Annexin V+/PI+); and dead cells are in the upper left quadrant (Annexin V-/PI+). (B) Represents the live and apoptotic beta cell population after treatment with cytokines and bergenin. Data are represented the mean ± standard deviation of 2 independent experiments. * indicates p <0.05, ‡<0.01 and § <0.001 relative to cytokine-treated cells.