Bergenin protects pancreatic beta cells against cytokine-induced apoptosis in INS-1E cells
Fig 3
Cellular effects of bergenin on mitochondrial physiological parameters of beta cells in the presence of cytokines.
Effects of bergenin on mitochondrial membrane potential (ΔΨm), intracellular ROS production, and mitochondrial dehydrogenase activity (MDA) after 48 hr treatment with IL-1β, INF-γ, and TNF-α. For dose-response studies, INS-1E cells were treated with various concentrations of bergenin (0.25–10 μM). The treated INS-1E cells were assessed for their ability to restore mitochondrial membrane potential (ΔΨm) (A); to reduce cellular ROS production (B); and to improve mitochondrial dehydrogenase activity (C). Data are represented as the mean ± standard deviation of 12 independent wells for A-F. * indicates p <0.05, ‡<0.01, and §<0.001 relative to cytokine-treated cells.