Specific induction and long-term maintenance of high purity ventricular cardiomyocytes from human induced pluripotent stem cells
Fig 5
Electrophysiological characterization and maturation of CMs after long-term culture.
(A) Criteria for CM subtype classification. (B) Relationship between APD30-40/APD70-80 ratio and dV/dt max on d21 and d91 CMs. (C) Pie charts illustrating the distribution of early ventricular-, late ventricular-, atrial- and nodal-like APs on d21 (n = 31 from three independent experiment), and d91 (n = 23 from three independent experiment). (D) Representative action potentials of early and late ventricular-like and nodal-like CMs. (E) Comparison of action potential parameters between early ventricular-like and late ventricular-like CMs on d21(n = 28 from three independent experiments) and d91 (n = 22 from three independent experiments). (n = 3). *p < 0.05, ***p < 0.001 by one-way ANOVA followed by Tukey-Kramer post hoc test. (F) Administration of E4031 (Ikr blocker) prolonged FPD in a dose-dependent manner at 0–30 nM in CMs. (G) Administration of Chromanol (Iks blocker) prolonged FPD in a dose-dependent manner at 0–30 μM in CMs. (H) Homogeneous response to Chromanol in a time-dependent manner. d31 (n = 16), over d91 (n = 8).