A protease protection assay for the detection of internalized alpha-synuclein pre-formed fibrils
Fig 5
Exposure of cells to extracellular TEV protease is not cytotoxic.
Neuro2A cells were exposed to TEV-P for 30 min and cell viability measured following an overnight growth period using the WST-1 cytotoxicity assay. Percent survival is plotted for treatment with TEV-P; 1% Triton X-100; 1x TEV-P buffer; and for untreated cells, by normalizing TEV-treated cell absorbance values to untreated cell absorbance values. TEV-P treatment does not result in significant cell death when compared to either buffer-treated or untreated cells. (****, p<0.0001; ns, not significant; one-way ANOVA and Tukey’s multiple comparisons tests).