A protease protection assay for the detection of internalized alpha-synuclein pre-formed fibrils
Fig 2
Characterization of α-syn-TEV-GFP pre-formed fibrils and seeding efficacy.
(Panel A) Electron microscopy images of negatively-stained α-synuclein monomers, intact fibrils, sonicated fibrils, and sonicated fibrils following TEV treatment. (Scale bar = 200 nm). (Panel B) Histogram of fibril size distribution after sonication with and without TEV treatment (bin size 20 nm; >200 fibrils counted for each condition). (Panel C) α-syn-TEV-GFP PFFs are competent to seed the fibrillation of α-synuclein monomers. Thioflavin T fluorescence, generated following fibril formation, is shown.