The effects of PEGylation on LNP based mRNA delivery to the eye
Fig 5
The effects of LNP size and cholesterol modifications post-intravitreal injection.
(A) Representative images demonstrating luciferase activity in the eye 24 hours post-injection. (B) Luciferase activity plotted for each group over time. Data are presented as mean ± SD. A two-way ANOVA, Tukey’s multiple comparisons test was used for analysis. *p<0.05. n = 3–6. (C) Representative bright field (top) and tdTomato (bottom) fundus images for each group taken 7 days post-injection. (D) Quantification of tdTomato intensity, represented as a fold change compared to PBS. Data are presented as mean ± SD. An ordinary one-way ANOVA, with Tukey’s correction for multiple comparisons test was used for comparisons. n = 3–6. (E) Representative confocal images of immunohistochemistry showing RFP expression in the Müller glia, the ONH and the TM for all groups. n.s.—not significant, LNP- lipid nanoparticle, RFP-red fluorescent protein, ONL- outer nuclear layer, INL-inner nuclear layer, GCL- ganglion cell layer, ONH-optic nerve head, TM-trabecular meshwork, CB-ciliary body.