A highly active heparinase I from Bacteroides cellulosilyticus: Cloning, high level expression, and molecular characterization
Fig 2
Effect of induction temperature on the activity (A) and the expression (B) (SDS-PAGE image of the BcHep-I at different temperatures.
M: protein molecular weight marker, lane 1~6: supernatant after the sonication of the IPTG induced E. coli at 15, 20, 25, 30, 35, and 37°C; lane 7~12: precipitate after the sonicated of the IPTG induced E. coli at 15, 20, 25, 30, 35, and 37°C; BcHep-I bands were marked with solid red wireframe) of BcHep-I. Effect of the induction time on the activity of BcHep-I (C). SDS-PAGE of the purified Hep-I (D) (M: protein molecular weight marker, lane 1: the purified BcHep-I).