The BBSome restricts entry of tagged carbonic anhydrase 6 into the cis-flagellum of Chlamydomonas reinhardtii
Fig 6
Schematic summary and model of expression regulation and flagellar localization of CAH6-mNG in C. reinhardtii.
A) CAH6 in flagella is actively converting carbon dioxide to bicarbonate and vice versa. CAH6 is predicted to attach to the flagellar membrane (pink) by a dual fatty acid modification (indicated by two wavy lines). B) In CO2-limiting conditions, the amount of CAH6 in flagella is upregulated 2- to 10-fold. C, D) The BBSome largely prevents the entry of CAH6-mNG into the cis-flagellum. In a hypothetical scenario, the younger basal body could recruit BBSomes to a specific location or activate the BBSome (indicated by the dark red color) to minimize entry of CAH6-mNG into the cis-flagellum; during maturation, the older basal body lost its ability to modulate BBSome function and CAH6-mNG will enter the flagella.