LPCAT1 enhances castration resistant prostate cancer progression via increased mRNA synthesis and PAF production
Fig 2
LPCAT1 overexpression alters motility in CRPC cells.
(A): LPCAT1 vector and empty vector (control) was transfected into C4-2 cells, and the transfection efficiency was demonstrated. (B): the ability of cell migration and invasion was determined at 0, 24 and 48 h after transfection. (C): The PAF concentration in cell cultures of LPCAT1 group and control group. (D) and (E): Cell migration and invasion assay were measured at 48 h after increasing concentration of PAF-AH (1–50 μg/ml) and ABT-491(10−7–10−5 M) in LPCAT1 group and control group. Values are presented as mean percent control ± s.e.m or mean ± s.e.m. * P < 0.05 and ** P < 0.01 compared with control.