Correlative fluorescence microscopy, transmission electron microscopy and secondary ion mass spectrometry (CLEM-SIMS) for cellular imaging
Fig 3
Detailed views of CLEM-SIMS in cell culture.
a) An individual cell was imaged in light microscopy, TEM, and nanoSIMS. The fluorescence channels show the uranyl acetate fluorescence (blue) and the MitoTracker fluorescence (magenta). The measured isotopes are indicated in the different nanoSIMS images. Nitrogen isotopes (14N, 15N) are measured as CN- ions, while carbon isotopes (12C, 13C) are measured as C2- ions. 13C and 15N are rare isotopes, which explains the low intensity of the respective images. Scale bars: 2 μm. b) Higher zoom views of the areas marked by white squares in panel A. Correlations of fluorescence (light) microscopy (left panels), TEM (middle panels) and nanoSIMS (right panels, 14N) are shown. Scale bars: 500 nm.