Correlative fluorescence microscopy, transmission electron microscopy and secondary ion mass spectrometry (CLEM-SIMS) for cellular imaging
Fig 2
A large field was imaged in both TEM and fluorescence, relying on the DAPI (blue) channel, which shows the uranyl acetate fluorescence, and on the mito-mCitrine channel (shown here in green). Scale bar: 10 μm. Boxed region 1 shows a higher zoom view on the light microscopy image as well as the correlated image with TEM of a cell marked by a white square in the overview image. Scale Bar: 2 μm. Boxed regions 2 and 3 show detailed images of CLEM and TEM in the regions of interest. Scale bar: 1 μm.