Sequence variation and immunogenicity of the Mycoplasma genitalium MgpB and MgpC adherence proteins during persistent infection of men with non-gonococcal urethritis
Fig 2
Immunoblot IgG reactivity of M. genitalium positive patient sera with whole lysates of strain G37.
Sera obtained from four PCR-positive men at first and third clinic visits (V1 and V3, respectively), were diluted 1:1,000 and reacted with M. genitalium whole cell lysates separated on a 7.5% SDS-PAGE gel. “Rb”, specific rabbit sera [40] was used to identify MgpB and MgpC protein bands (arrows). Molecular weight markers (in kDa) are shown at left. The magnitude of antibody reactivity between patients cannot be compared as different film exposure times were used.