Association of host proteins with the broad host range filamentous phage NgoΦ6 of Neisseria gonorrhoeae
Fig 5
Identification of protein glycosylation.
Proteins extracted from phage or phagemid particles were separated on a 5% to 15% SDS-PAGE gel and stained with Pro-Q Emerald 480 Glycoprotein Detection kit and then visualized with UV illumination. The lanes represent: MW, BioRad Dual Extra Molecular weight standards; Lane 1; phagemid pBSKS::Φ6fm isolated from E. Top10, lane 2; pBSKSΦ6::fm from S. enterica; lane 3 and 4; phage NgoΦ6; lane 5, pBSKS::Φ6fm isolated from H. influenzae. Equal amounts (10 μg) of phage proteins were loaded on the gel. The lanes M represent two proteins of molecular size 18 kDa and 42 kDa of CandyCane Glycoprotein Molecular Weight Standards stained by Emerald 488.