Kahweol activates the Nrf2/HO-1 pathway by decreasing Keap1 expression independently of p62 and autophagy pathways
Fig 5
The effect of kahweol (Kah) on Keap1 protein degradation.
(A) AML12 cells (left) were treated with 20 μM kahweol and primary hepatocytes (right) were treated with 40 μM kahweol in the absence or presence of E64d (10 μM), MG132 (0.5 μM), or chloroquine (CQ; 10 μM). Data in the bar graph are means ± SEM of three independent measurements. * p < 0.05 compared with the control. # p < 0.05. (B) Western blot analyses of Keap1 and HO-1 protein levels, in primary hepatocytes from ATG7f/f Alb-Cre+ (autophagy-deficient) mice. Data in the bar graph are means ± SEM of three independent measurements. * p < 0.05 compared with the ATG7f/f, # p < 0.05 compared with the ATG7f/f-Cre+.