Kahweol activates the Nrf2/HO-1 pathway by decreasing Keap1 expression independently of p62 and autophagy pathways
Fig 4
The effect of kahweol (Kah) on p62 expression.
AML12 cells were treated with 20 μM kahweol and primary hepatocytes were treated with 40 μM kahweol. (A,B) Real-time RT-PCR (A) and western blot (B) analyses of p62 mRNA and protein levels, respectively, in AML12 cells (left) and primary hepatocytes (right). Data in the bar graph are means ± SEM of three independent measurements; * p < 0.05 compared with the control for each time. (C) Primary hepatocytes were transfected with a p62-specific siRNA (siRNA-p62; 100 nM) or a control siRNA (siCon; 100 nM), treated with 40 μM kahweol for 24 h, and then analyzed by western blotting. Data in the bar graph are means ± SEM of three independent measurements. * p < 0.05 compared with the control siRNA. # p < 0.05 compared with the siRNA-p62.