Kahweol activates the Nrf2/HO-1 pathway by decreasing Keap1 expression independently of p62 and autophagy pathways
Fig 3
The effect of kahweol (Kah) on Keap1 expression.
AML12 cells were treated with 20 μM kahweol and primary hepatocytes were treated with 40 μM kahweol. (A, B) Real-time RT-PCR (A) and western blot (B) analyses of Keap1 mRNA and protein levels, respectively, in AML12 cells (left) and primary hepatocytes (right). Data in the bar graphs are means ± SEM of three independent measurements. * p < 0.05 compared with the control for each time.