Kahweol activates the Nrf2/HO-1 pathway by decreasing Keap1 expression independently of p62 and autophagy pathways
Fig 1
The effects of kahweol on H2O2-induced oxidant stress in hepatocytes.
AML12 cells were treated with kahweol (10 or 20 μM) for 19 h and then incubated with H2O2 (1mM) for a further 5 h. (A) DCF-DA staining of cells treated with 1 mM H2O2 and with or without 10 μM kahweol. Data in the bar graph are means ± SEM of three independent measurements. * p < 0.05 compared with the control, # p < 0.05 compared with the H2O2 treatment. (B) The viability of AML12 cells treated with or without H2O2 and kahweol, as determined by a CCK-8 assay. (C) FACScan flow cytometry analyses of AML12 cells treated with or without H2O2 and kahweol. Apoptosis was determined based on the sub-G1 population. (D) Western blot analyses of cleaved caspase 3 and HO-1 levels in AML12 cells treated with or without kahweol (10 or 20 μM) and 1 mM H2O2. * p < 0.05 compared with the control, # p < 0.05 compared with the H2O2 treatment. Con, control; Kah, kahweol.