Establishment and characterization of VOA1066 cells: An undifferentiated endometrial carcinoma cell line
Fig 5
Mutations of DICER1 genes in DDEC.
(A) Sanger sequencing validated the DICER1 p.D1810Y mutation in both DNA and RNA samples of VOA1066 cells. (B) MicroRNA analyses in VOA1066 cells and SVOG3e cells, a DICER1-wildtype granulosa cell line. (C) Mutational analysis of DICER1 RNase IIIb domain in additional 33 DDEC cases. (D) IHC analysis of SWI/SNF proteins (SMARCA4, SMARCB1, ARID1A, ARID1B) in 6 cases of endometrial cancer with DICER1 RNase IIIb domain hotspot mutations at Vancouver General Hospital (VGH) and mutational analyses of 17 cases of TCGA endometrial cancers with DICER1 RNase IIIb domain hotspot mutations.