The vimentin rod domain blocks P-selectin-P-selectin glycoprotein ligand 1 interactions to attenuate leukocyte adhesion to inflamed endothelium
Fig 2
SPOT-peptide membranes of (A) full-length human vimentin and (B) human P-selectin. The dark spots indicate locations in which IR800-labeled proteins bound to 20 amino acid residues from (A) full-length vimentin or (B) P-selectin. (A) IR-labeled P-sel/Fc bound to 20 amino acid spots of full-length vimentin that are located primarily in the rod domain. There is an 18-aa overlap between adjacent spots. (B) IR800-labled rhRod bound to 20 amino acid spots from P-selectin. There is a 17-aa overlap between adjacent spots. Letters and numbers correlate to the 20-aa sequence list in S1 Table (full-length vimentin) & S2 Table (P-selectin).