A novel small molecule LLL12B inhibits STAT3 signaling and sensitizes ovarian cancer cell to paclitaxel and cisplatin
Fig 2
LLL12B inhibits p-STAT3 and its downstream targets in human ovarian cancer cells.
A. The effects of LLL12B on STAT3 phosphorylation (Tyr705) and its downstream targets in ovarian cancer cell lines. A2780, CAOV3, SKOV3, OVCAR5 cells were treated with DMSO or different concentrations of LLL12B. The levels of p-STAT3 and the downstream target gene proteins were determined by Western blots. B. Compared with cisplatin or paclitaxel alone, the combination of LLL12 with cisplatin or/and paclitaxel appeared to have a greater inhibitory effect on p-STAT3 and some downstream target gene proteins in CAOV3, SKOV3 and OVCAR5 cells. C. Treated with LLL12B by 0h,2h and 4h, p-STAT3 was inhibited in four cell lines, p-Jak1 was only inhibited in A2780; p-Jak2 was not detected. Total Jak1, Jak2 in SKOV3 was reduced. The total STAT3 and total Jak1, Jak2 in other three cell lines were not reduced.