Elevated miR-16-5p induces somatostatin receptor 2 expression in neuroendocrine tumor cells
Fig 2
Microarray analysis of miRNA expression.
miRNA profiling was performed to determine the (A) number and (B) profile of differentially expressed miRNAs. Red and green indicate significantly upregulated or downregulated miRNAs, respectively. INS-1 cells (C) and GH3 cells (D) were incubated with 1 μmol/L octreotide. After the indicated incubation time, total RNA was isolated, after which miR-16-5p levels were measured by qRT-PCR and normalized for input RNA using control miRNA (U87) with the 2−ΔΔCt method. Data represent the mean of three independent experiments ± SD; *p < 0.05, **p < 0.01.