Thermal cycling protects SH-SY5Y cells against hydrogen peroxide and β-amyloid-induced cell injury through stress response mechanisms involving Akt pathway
Fig 9
Effect of LY294002 on the neuroprotective effect of TC-HT and related protein expressions.
(A) TC treatment conferred neuroprotective effect and significantly increased the cell viability of SH-SY5Y neuron cells under the H2O2 stress. The neuroprotective effect of TC treatment was abrogated by addition of the PI3K inhibitor LY294002 in a dose-dependent manner. (B) Cells were lysed 15 h after H2O2 or 25 μM LY294002 treatment, and western blot analyses of p-Akt and Nrf2 proteins were performed. The inhibitor LY294002 reversed the activated levels of p-Akt and Nrf2 induced by TC treatment. The expression level of p-Akt and Nrf2 was normalized to total Akt and GAPDH, respectively. We used the abbreviation “LY” to represent the PI3K inhibitor LY294002 in the figure. Data represent the mean ± standard deviation (n = 3). ***P < 0.001, **P < 0.01 and *P < 0.05.