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Thermal cycling protects SH-SY5Y cells against hydrogen peroxide and β-amyloid-induced cell injury through stress response mechanisms involving Akt pathway

Fig 5

Effect of TC-HT on H2O2-induced MMP reduction in SH-SY5Y cells.

(A) MMP was analyzed 24 h after the H2O2 treatment by flow cytometry with DiOC6(3) fluorescent dye. (B) Quantification of the cells with decreased MMP after H2O2, TC+H2O2, or HT+H2O2 treatment. Data represent the mean ± standard deviation (n = 3). ***P < 0.001 and **P < 0.01.

Fig 5

doi: https://doi.org/10.1371/journal.pone.0240022.g005