Thermal cycling protects SH-SY5Y cells against hydrogen peroxide and β-amyloid-induced cell injury through stress response mechanisms involving Akt pathway
Fig 2
Effect of TC-HT on H2O2-induced cytotoxicity in SH-SY5Y cells.
(A) Dose-response curve of SH-SY5Y cells treated with different concentrations of H2O2 for 24 h. (B) SH-SY5Y cells were pretreated at 41.5°C temperature setting and challenged with or without 450 μM H2O2. The cell viability was measured by MTT assay at 24 h after the H2O2 treatment. (C) SH-SY5Y cells were pretreated at 42.5°C temperature setting with different thermal dosages and challenged with or without 450 μM H2O2. The cell viability was measured by MTT assay at 24h after the H2O2 treatment. (D) Comparison of the neuroprotective effect under different low temperature period settings. (E) The LDH release was measured to confirm the neuroprotective effect of TC treatment. SH-SY5Y cells were pretreated at 42.5°C temperature setting and challenged with or without 450 μM H2O2. The LDH release was measured 24h after the H2O2 treatment. Data represent the mean ± standard deviation (n = 3). ***P < 0.001 and **P < 0.01.