Refining surgical models of osteoarthritis in mice and rats alters pain phenotype but not joint pathology
Fig 5
Quantification of neuroimmune cell activation in the dorsal horn of the spinal cord.
Adult male C57BL/6 mice underwent traditional DMM, modified DMM, or sham surgery. Dorsal horn spinal cord Iba-1 immunostaining for microglia and GFAP immunofluorescence for astrocytes was quantified at 16 weeks after surgery. (A) Quantification of Iba-1 immunostaining in mice that underwent traditional DMM (n = 8) or sham surgery (n = 6). (B) Quantification of IBA1 immunostaining in mice that underwent modified DMM (n = 8) or sham surgery (n = 6). Data are reported as average number of activated microglia in the ipsilateral and contralateral dorsal horn. (C) Representative images of Iba-1 staining in the dorsal horn of the spinal cord. Data were analysed by Kruskal-Wallis test with Dunn’s multiple comparisons, no significant differences observed. GFAP immunofluorescence data are the average sum grey intensity value measured using Velocity software. (D) Quantification of GFAP immunostaining in mice that underwent traditional DMM (n = 8) or sham surgery (n = 6). (E) Quantification of GFAP immunostaining in mice that underwent modified DMM (n = 8) or sham surgery (n = 6). (F) Representative images of GFAP immunostaining in the superficial dorsal horn of mice that underwent traditional or modified DMM surgery. Data were analysed by one-way ANOVA with a Dunnett’s multiple comparison, no significant differences observed.