Mining a human transcriptome database for chemical modulators of NRF2
Fig 2
Predictive accuracy of the NRF2 biomarker.
A. Predictions using the NRF2 biomarker were compared to those from the HTS NRF2 activation assays. The dashed line represents the cutoff value of four for significant biomarker activation. Those chemicals that were active (red) or inactive (black) in the HTS assays are shown. Three false positives (blue) and one false negative (yellow) are indicated. Inset, binary classifier calculations based on NRF2 HTS assay results. B. Expression changes of the biomarker genes for the three false positives and one false negative. (Top) The bar graph shows the -Log(p-values) of the comparisons between the biomarker and the indicated chemical. (Bottom) The heatmap shows the expression of the biomarker genes.